jeremy wilusz (Addgene inc)
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jeremy wilusz
Jeremy Wilusz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jeremy+wilusz/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/bio_rxiv__2025__10__19__682992-235-9-11
Average 96 stars, based on 247 article reviews
Jeremy Wilusz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jeremy+wilusz/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/bio_rxiv__2025__10__19__682992-235-9-11
Average 96 stars, based on 247 article reviews
jeremy wilusz - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Development of a trilipid-based liposome system as a delivery vector for plasmid DNA in an MCF-7 cell line: Preparation, optimization, physical characterization and In Vitro cytotoxicity evaluation Article Snippet: .. pcDNA3.1(+)eGFP was a gift from Article Title: RNA editing enzyme ADAR2 regulates P-glycoprotein expression in murine breast cancer cells through the circRNA-miRNA pathway. Article Snippet: The following reagents and equipment were used: PrimeSTAR MAX DNA polymerase (Takara Bio Inc., Shiga, Japan), pGL4.13 luciferase reporter vector, pmirGLO vector, pRL-TK vector, Dual-Luciferase reporter assay system, GloMax 20/20 Luminometer (Promega, Madison, WI), miRIDIAN microRNA Mimic Negative Control #1 and mmu-miR-195a-3p (Horizon Discovery, Cambridge, UK), Lipofectamine LTX reagent, and Lipofectamine 2000 reagent (Invitrogen; Life Technologies). .. The pcDNA3.1(+) CircRNA Mini Vector was a gift from Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Article Title: CAG repeat expansions create splicing acceptor sites and produce aberrant repeat-containing RNAs. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1 ATXN8KKQ*AS-AmpR AUG 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR stop 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR frameshift 1003CAG This paper Table S4 pHR ATNX8 400bp endogenous sequence This paper Table S4 pHR ATXN8*donor 400bp endogenous sequence This paper Table S4 pHR ATXN8*AUG 400bp endogenous sequence This paper Table S4 pcDNA3.1 EGFP, vector control Xiao et al.66 Addgene plasmid #129020 pCMV-VSV-G, Lentivirus packaging Stewart et al.67 Addgene plasmid #8454 psPAX2, Lentivirus packaging n/a Addgene plasmid #12260 pX330-U6-Chimeric_BB-CBh-hSpCas9 Cong et al.68 Addgene plasmid #42230 Software and algorithms Prism v10.0.3 GraphPad RRID:SCR_002798; http://www.graphpad.com/ ImageJ v1.53q Schneider et al.69 RRID:SCR_003070; https://imagej.net/ samtools v1.11 Danecek et al.70 RRID:SCR_002105; http://www.htslib.org/ BBTools v38.86 Brian Bushnell RRID:SCR_016968; https://sourceforge. .. Lentiviral transfer plasmids with CAG-repeats and MS2-hairpins were previously described,5 as were the plasmids with 2403CAG repeats with various upstream flanking sequences (including CAGRAN and CAGFOCI).12 Plasmids with 110 CAG repeats and endogenous flanking sequences from ATXN8, JPH3, DMPK, HTT, and ATXN3 were a generous gift from Dr. Laura Ranum.10 pcDNA3.1(+) EGFP was a gift from Article Title: Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Article Snippet: Expression of miR-874-3p was detected using miR-874-3p primer purchased fromApplied Biosystems or Qiagen. .. The primer sequences are summarized in Table S3. circSmoc1-2 cloning The full circSmoc1-2 sequence was cloned into a pcDNA3.1(+) Laccase2 MCS exon vector,26 a gift from Article Title: circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA Article Snippet: The amplicons were inserted into pcDNA5- Twin- Streptag. pcDNA5- Twin- Streptag was made by inserting a PCR product (containing a Twin- Streptag followed by a TEV protease site) using a pDSG- IBA- Twin- Strep- Tag vector as template (IBA- lifesciences) into the HindIII–ApaI site of pcDNA5- FRT- TO (Invitrogen). .. To generate plasmids for exogenous expression of circHIPK3, the second exon encoding circHIPK3 was PCR amplified from cDNA prepared from RNA isolated from HEK293/HeLa cells and inserted into PacI/SacII- digested pcDNA3.1(+)- Laccase2- MCS- exon- vector (Kramer et al., 2015) to generate the pcDNA3.1(+)- Laccase2- circHIPK3 vector. pcDNA3.1(+) Laccase2- MCS- Exon- Vector was a gift from Article Title: CircNFATc3 promotes Fetal Hemoglobin induction by regulating let-7b/GATA2 axis Article Snippet: Images of the stained cells were taken using Nikon TE2000 microscope equipped with a digital camera. .. The pcDNA3.1(+) CircRNA Mini Vector was a gift from Article Title: Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells. Article Snippet: .. Fluorescent proteins were amplified from pcDNA3.1(+) eGFP, a gift from Over Expression:Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Article Title: Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Article Snippet: Expression of miR-874-3p was detected using miR-874-3p primer purchased fromApplied Biosystems or Qiagen. .. The primer sequences are summarized in Table S3. circSmoc1-2 cloning The full circSmoc1-2 sequence was cloned into a pcDNA3.1(+) Laccase2 MCS exon vector,26 a gift from Construct:Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Cloning:Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Article Title: Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Article Snippet: Expression of miR-874-3p was detected using miR-874-3p primer purchased fromApplied Biosystems or Qiagen. .. The primer sequences are summarized in Table S3. circSmoc1-2 cloning The full circSmoc1-2 sequence was cloned into a pcDNA3.1(+) Laccase2 MCS exon vector,26 a gift from Luciferase:Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Sequencing:Article Title: CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein Article Snippet: All cell lines were verified by short tandem repeat profiling at the GENEWIZ, Inc. within 6 months of use, and were cultured under the condition as described previously [ ]. .. CircRNA overexpression plasmid was constructed by cloning the RTN4 exon 4 and 5 into pcDNA3.1 (+) circRNA mini vector, which was a gift from Article Title: Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Article Snippet: Expression of miR-874-3p was detected using miR-874-3p primer purchased fromApplied Biosystems or Qiagen. .. The primer sequences are summarized in Table S3. circSmoc1-2 cloning The full circSmoc1-2 sequence was cloned into a pcDNA3.1(+) Laccase2 MCS exon vector,26 a gift from Polymerase Chain Reaction:Article Title: CAG repeat expansions create splicing acceptor sites and produce aberrant repeat-containing RNAs. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1 ATXN8KKQ*AS-AmpR AUG 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR stop 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR frameshift 1003CAG This paper Table S4 pHR ATNX8 400bp endogenous sequence This paper Table S4 pHR ATXN8*donor 400bp endogenous sequence This paper Table S4 pHR ATXN8*AUG 400bp endogenous sequence This paper Table S4 pcDNA3.1 EGFP, vector control Xiao et al.66 Addgene plasmid #129020 pCMV-VSV-G, Lentivirus packaging Stewart et al.67 Addgene plasmid #8454 psPAX2, Lentivirus packaging n/a Addgene plasmid #12260 pX330-U6-Chimeric_BB-CBh-hSpCas9 Cong et al.68 Addgene plasmid #42230 Software and algorithms Prism v10.0.3 GraphPad RRID:SCR_002798; http://www.graphpad.com/ ImageJ v1.53q Schneider et al.69 RRID:SCR_003070; https://imagej.net/ samtools v1.11 Danecek et al.70 RRID:SCR_002105; http://www.htslib.org/ BBTools v38.86 Brian Bushnell RRID:SCR_016968; https://sourceforge. .. Lentiviral transfer plasmids with CAG-repeats and MS2-hairpins were previously described,5 as were the plasmids with 2403CAG repeats with various upstream flanking sequences (including CAGRAN and CAGFOCI).12 Plasmids with 110 CAG repeats and endogenous flanking sequences from ATXN8, JPH3, DMPK, HTT, and ATXN3 were a generous gift from Dr. Laura Ranum.10 pcDNA3.1(+) EGFP was a gift from Article Title: circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA Article Snippet: The amplicons were inserted into pcDNA5- Twin- Streptag. pcDNA5- Twin- Streptag was made by inserting a PCR product (containing a Twin- Streptag followed by a TEV protease site) using a pDSG- IBA- Twin- Strep- Tag vector as template (IBA- lifesciences) into the HindIII–ApaI site of pcDNA5- FRT- TO (Invitrogen). .. To generate plasmids for exogenous expression of circHIPK3, the second exon encoding circHIPK3 was PCR amplified from cDNA prepared from RNA isolated from HEK293/HeLa cells and inserted into PacI/SacII- digested pcDNA3.1(+)- Laccase2- MCS- exon- vector (Kramer et al., 2015) to generate the pcDNA3.1(+)- Laccase2- circHIPK3 vector. pcDNA3.1(+) Laccase2- MCS- Exon- Vector was a gift from Mutagenesis:Article Title: CAG repeat expansions create splicing acceptor sites and produce aberrant repeat-containing RNAs. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1 ATXN8KKQ*AS-AmpR AUG 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR stop 1003CAG This paper Table S4 pcDNA3.1 ATXN8KKQ,AS-AmpR frameshift 1003CAG This paper Table S4 pHR ATNX8 400bp endogenous sequence This paper Table S4 pHR ATXN8*donor 400bp endogenous sequence This paper Table S4 pHR ATXN8*AUG 400bp endogenous sequence This paper Table S4 pcDNA3.1 EGFP, vector control Xiao et al.66 Addgene plasmid #129020 pCMV-VSV-G, Lentivirus packaging Stewart et al.67 Addgene plasmid #8454 psPAX2, Lentivirus packaging n/a Addgene plasmid #12260 pX330-U6-Chimeric_BB-CBh-hSpCas9 Cong et al.68 Addgene plasmid #42230 Software and algorithms Prism v10.0.3 GraphPad RRID:SCR_002798; http://www.graphpad.com/ ImageJ v1.53q Schneider et al.69 RRID:SCR_003070; https://imagej.net/ samtools v1.11 Danecek et al.70 RRID:SCR_002105; http://www.htslib.org/ BBTools v38.86 Brian Bushnell RRID:SCR_016968; https://sourceforge. .. Lentiviral transfer plasmids with CAG-repeats and MS2-hairpins were previously described,5 as were the plasmids with 2403CAG repeats with various upstream flanking sequences (including CAGRAN and CAGFOCI).12 Plasmids with 110 CAG repeats and endogenous flanking sequences from ATXN8, JPH3, DMPK, HTT, and ATXN3 were a generous gift from Dr. Laura Ranum.10 pcDNA3.1(+) EGFP was a gift from Clone Assay:Article Title: Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Article Snippet: Expression of miR-874-3p was detected using miR-874-3p primer purchased fromApplied Biosystems or Qiagen. .. The primer sequences are summarized in Table S3. circSmoc1-2 cloning The full circSmoc1-2 sequence was cloned into a pcDNA3.1(+) Laccase2 MCS exon vector,26 a gift from Expressing:Article Title: circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA Article Snippet: The amplicons were inserted into pcDNA5- Twin- Streptag. pcDNA5- Twin- Streptag was made by inserting a PCR product (containing a Twin- Streptag followed by a TEV protease site) using a pDSG- IBA- Twin- Strep- Tag vector as template (IBA- lifesciences) into the HindIII–ApaI site of pcDNA5- FRT- TO (Invitrogen). .. To generate plasmids for exogenous expression of circHIPK3, the second exon encoding circHIPK3 was PCR amplified from cDNA prepared from RNA isolated from HEK293/HeLa cells and inserted into PacI/SacII- digested pcDNA3.1(+)- Laccase2- MCS- exon- vector (Kramer et al., 2015) to generate the pcDNA3.1(+)- Laccase2- circHIPK3 vector. pcDNA3.1(+) Laccase2- MCS- Exon- Vector was a gift from Amplification:Article Title: circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA Article Snippet: The amplicons were inserted into pcDNA5- Twin- Streptag. pcDNA5- Twin- Streptag was made by inserting a PCR product (containing a Twin- Streptag followed by a TEV protease site) using a pDSG- IBA- Twin- Strep- Tag vector as template (IBA- lifesciences) into the HindIII–ApaI site of pcDNA5- FRT- TO (Invitrogen). .. To generate plasmids for exogenous expression of circHIPK3, the second exon encoding circHIPK3 was PCR amplified from cDNA prepared from RNA isolated from HEK293/HeLa cells and inserted into PacI/SacII- digested pcDNA3.1(+)- Laccase2- MCS- exon- vector (Kramer et al., 2015) to generate the pcDNA3.1(+)- Laccase2- circHIPK3 vector. pcDNA3.1(+) Laccase2- MCS- Exon- Vector was a gift from Article Title: Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells. Article Snippet: .. Fluorescent proteins were amplified from pcDNA3.1(+) eGFP, a gift from Isolation:Article Title: circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA Article Snippet: The amplicons were inserted into pcDNA5- Twin- Streptag. pcDNA5- Twin- Streptag was made by inserting a PCR product (containing a Twin- Streptag followed by a TEV protease site) using a pDSG- IBA- Twin- Strep- Tag vector as template (IBA- lifesciences) into the HindIII–ApaI site of pcDNA5- FRT- TO (Invitrogen). .. To generate plasmids for exogenous expression of circHIPK3, the second exon encoding circHIPK3 was PCR amplified from cDNA prepared from RNA isolated from HEK293/HeLa cells and inserted into PacI/SacII- digested pcDNA3.1(+)- Laccase2- MCS- exon- vector (Kramer et al., 2015) to generate the pcDNA3.1(+)- Laccase2- circHIPK3 vector. pcDNA3.1(+) Laccase2- MCS- Exon- Vector was a gift from |